Your browser does not support JavaScript!
National Yang-Ming University, Department & Institute of Physiology
National Yang-Ming University, Department & Institute of Physiology
Tzong-Shyuan Lee

 Research Field    Publications    Research grant    Lab intro    (2016.06.29 update)


Tzong-Shyuan Lee





Tel. 886-2-2826-7365
Fax No. 886-2-2826-4049




Graduate Institute of Life Sciences, National Defense Medical Center, Taipei, Taiwan, R.O.C.



Department of Veterinary Medicine, National Chung-Hsing University, Taichung, Taiwan, R.O.C.



Department of Veterinary Medicine, National Chung-Hsing University, Taichung, Taiwan, R.O.C.


Assistant Research Fellow (1974-1975), Institute of Zoology, Academia Sinica Taipei, Taiwan,ROC

Research Interests:

Topics of research in my lab are:

1. Role and pathophysiological mechanisms of soluble epoxide hydrolase (sEH) in inflammatory diseases and neuropsychiatric disorders.

2. Role erythropoietin (EPO) in the deregulation of lipid metabolism- related diseases and neuropsychiatric disorders.

3. The pathophysiology of TRP channels in lipid metabolism- related diseases and neuropsychiatric disorders.

4. Beneficial effects and its underlying molecular mechanisms of dietary supplements in the prevention or treatment of atherosclerosis.

We apply various techniques of cellular and molecular biology including primary cell culture, western blotting, immunoprecipitation, paraffin section, frozen section, immunohistochemistry, RT-PCR, real time PCR, microarray, gene cloning, gene transfection, adenovirus infection and transgenic mice in our studies.

Currently (2013), my lab includes 4 students in the Master program, 4 students in the Ph.D. program, and 1 full-time postdoctoral fellow and 1 technician. I also collaborate with professors in Yang-Ming University, Taipei Veterans General Hospital, Cheng-Hsin General Hospital and Institute of Biomedical Sciences, Academic Sinica, Taiwan.


Selected Recent Publications:
1 James A. Lin, C.-L., Shyu, and Tzong-Shyuan Lee. 1994. Establishment of Mycoplasma spp. cleaning program in a breeder farm combined with Ofloxacin. Taiwan J. Vet. Med. Ani. Husb. 64: 67-76.
James A. Lin, Tzong-Shyuan Lee, J.-S. Wang and K.-T. Tong. 1995. The artifical cryptosporidal infection on immunosuppression of Marek’s disease. Taiwan J. Vet. Med. Anim. Husb. 65: 147-152.
3 James A. Lin and Tzong-Shyuan Lee. 1995. The application of ELISA for detecting antibody to avian influenza virus. J. Chin. Soc. Vet. Sci. 21: 146-151.
James A. Lin and Tzong-Shyuan Lee. 1996. Genetic susceptibility to Marek's disease virus of local chickens in Taiwan. Avian Dis. 40: 576-581.
5 James A. Lin and Tzong-Shyuan Lee. 1996. Protectivity of the bivalent Marek's disease vaccines in Taiwan local chickens. J. Chin. Soc. Vet. Sci. 22: 107-112.
6 Li-Ju Wang, Tzong-Shyuan Lee, Fan-Yen Lee, Ru-Chun Pai, and Lee-Young Chau. 1998. Expression of heme oxygenase-1 in atherosclerotic lesions. Am. J. Pathol. 152: 711-720.
7 Fan-Yen Lee, Tzong-Shyuan Lee, Ching-Chien Pan, An-Li Huang, and Lee-Young Chau. 1998. Colocalization of iron and ceroid in human atherosclerotic lesions. Atherosclerosis 138: 281-288.
8 Tzong-Shyuan Lee, Hwei-Chen Yen, Ching-Chien Pan, and Lee-Young Chau. 1999. The role of interleukin 12 in the development of atherosclerosis in apoE-deficient mice. Arterioscler. Thromb. Vasc. Biol. 19: 734-742.
9 Tzong-Shyuan Lee, Ming-Shi Shiao, Ching-Chien Pan, and Lee-Young Chau. 1999. Iron-deficient diet reduces atherosclerotic lesions in apoE-deficient mice. Circulation 99: 1222-1229.
10 Tzong-Shyuan Lee, Fan-Yen Lee, Jong-Hwei S. Pang, and Lee-Young Chau. 1999. Erythrophagocytosis and iron deposition in atherosclerotic lesions. Chin. J. Physiology 41: 17-23.
11 Tzong-Shyuan Lee and Lee-Young Chau. 2001. Fas/Fas ligand mediated death pathway is involved in oxide LDL-induced apoptosis in vascular smooth muscle cells. Am. J. Physiol. Cell Physiol. 280:C709-C718.
12 Shu-Hui Juan*, Tzong-Shyuan Lee*, Kuang-Wen Tseng, Jun-Yang Liou, Song-Kun Shyue, Kenneth K. Wu, Lee-Young Chau. 2001. Adenovirus-mediated heme oxygenase-1 gene transfer inhibits the development of atherosclerosis in ApoE-deficient meic. Circulation 104:1519-1525.(*these authors contributed equally to this work).
13 Tzong-Shyuan Lee and Lee-Young Chau. 2002. Heme oxygenase-1 mediates the anti-inflammatory effect of interleukin-10 in mice. Nature Med. 8: 240-246.
14 Tzong-Shyuan Lee, Hui-Ling Tsai and Lee-Young Chau. 2003. Induction of heme oxygenase-1 expression in murine macrophage is essential for the anti-inflammatory effect of low dose 15-deoxy-D12,14-prostaglandin J2. J. Biol. Chem. 278: 19325-19330.
15 HT Lee, LL Chiu, Tzong-Shyuan Lee, Hui-Ling Tsai, Lee-Young Chau.  2003. Dietary iron restriction increases plaque stability in apolipoprotein- E-deficient mice. J. Biomed. Sci. 10:510-517
16 Rui-Hai Zhou, Tzong-Shyuan Lee, Tsui-Chun Tsou, Francois Rannou, Yi-Shuan Li, Shu Chien, John Y.-J Shyy. 2003. Stent Implantation activates Akt in the vessel wall. Role of mechanical stretch in vascular smooth muscle cells.  Arterioscler Thromb Vasc Biol. 23: 2015-2020.
17 Francois Rannou, Tzong-Shyuan Lee, Rui-Hai Zhou, Jennie Chin, Jeffrey C   Lotz, Marie-Anne Mayous-Benhamou, Jacques Patrick Barbet, Alain Chevrot, and JY-J. Shyy. 2004. Intervertebral disc degeneration: The role of the mitochondrial pathway in annulus fibrosus cell apoptosis induced by overload. Am. J. Pathol. 164: 915-924.
18 Yi Liu, Yi Zhu, Francois Rannou, Tzong-Shyuan Lee, Kitty Formentin, Lingfang Zeng, Xiaohui Yuan, Nanping Wang, Shu Chien, Barry M. Forman, and John Y-J. Shyy. 2004. Laminar flow activates peroxisome proliferator-activator receptor . Circulation 110: 1128-1133.
19 Rui-Hai Zhou, Yao Min, Tzong-Shyuan Lee, Yi Zhu, Martins-Green Manuela, John Y-J. Shyy. 2004. Vascular endothelial growth factor activation of sterol regulatory element binding protein. A potential role in angiogenesis. Circ Res. 95:471-478.
20 Tzong-Shyuan Lee, Chih-Chieh Chang, Yi Zhu, and John Y-J. Shyy. 2004. Simvastatin induction of heme oxygenase-1: A novel mechanism of vessel protection. Circulation 110: 1296-1302.
21 Lingfang Zeng, Hailing Liao, Yi Liu , Tzong-Shyuan Lee, Minjia Zhu, Xian Wang, Michael B Stemerman, Yi Zhu, John Y-J Shyy. 2004. SREBP2 downregulates ABCA1 in vascular endothelial cells: a novel role of SREBP in regulating cholesterol metabolism. J. Biol. Chem. 279:48801-48807.
22 Yi Zhu, Hailing Liao, Xuefen Xie, Yuan Yuan, Tzong-Shyuan Lee, Nanping Wang, Xian Wang, John Y.-J. Shyy and Michael B. Stemerman. 2005. Oxidized LDL downregulates ATP-binding cassette transporter-1 in human vascular endothelial cells via inhibiting liver X receptor (LXR). Cardiovasc Res. 68: 425-432.
23 Yi Liu , Yingjia Zhang , Kara Schmelzer, Tzong-Shyuan Lee, Xiang Fang , Yi Zhu, Arthur A. Spector, Sarjeet Gill, Christophe Morisseau, Bruce D. Hammock and John Y.-J. Shyy. 2005. The anti-inflammatory effect of laminar flow: The role of PPARg, EETs and sEH. Proc Natl Acad Sci U S A. 102:16747-16752.
24 Yingjia Zhang, Tzong-Shyuan Lee, Erik M. Kolb; Kai Sun, Xiao Lu, Frances M. Sladek; Ghassan S. Kassab; Theodore Garland, Jr; John Y.-J. Shyy. 2006. AMP-Activated Protein Kinase Is Involved in Endothelial NO Synthase Activation in Response to Shear Stress. Arterioscler Thromb Vasc Biol. 26:1281-1287.
25 Wei Sun*, Tzong-Shyuan Lee*, Minjia Zhu, Yi Zhu, John YJ Shyy. 2006. Statins activate AMP-activated kinase in vitro and in vivo. Circulation. 114: 2655-2662. (*these authors contributed equally to this work).
26 You-Lan Yang, Gau-Jun Tang, Yuh-Lin Wu, Huey-Wen Yien, Tzong-Shyuan Lee*, Yu Ru Kou*. 2008. Exacerbation of wood smoke-induced acute lung injury by mechanical ventilation using moderately high tidal volume in mice. Respiratory Physiology & Neurobiology. 2008. 160:99-108. (*Corresponding author)
27 Yuh-Lin Wu,  Hsiang-Hao Chuang, Ru Yu Kou, Tzong-Shyuan Lee, Shing-Hwa Lu, Yu-Chu Huang, Yoshihiro Nishi, Toshihiko Yanase. 2008. Regulation of LH Receptor and PGF2a Receptor Signaling by the Regulator of G Protein Signaling 2 (RGS2) in Human and Mouse Granulosa Cells. Chinese Journal of Physiology. 51: 1-10.
28 Tzong-Shyuan Lee, Yu-Ju Liu, Huey-Wen Yien, Yuh-Lin Wu, Yu Ru Kou. 2008. Wood smoke extract promotes both apoptosis and proliferation in rat alveolar epithelial type II cells: the role of oxidative stress and heme oxygenase-1. Crit Care Med. 36:2597-2606.
29 Ya-Shan Cheng, Tzong-Shyuan Lee, Hui-Chi Hsu, Yu Ru Kou, Yuh-Lin Wu. 2008. Characterization of the transcriptional regulation of the regulator of G protein signaling 2 (RGS2) gene during 3T3-L1 preadipocyte differentiation. J Cell Biochem. 105:922-930.
30 Tzong-Shyuan Lee, Chun-Yueh Lin, Jin-Yi Tsai, Yuh-Lin Wu, Kuo-Hui Su, Kuo-Yun Lu, Sheng-Huang Hsiao, Ching-Chian Pan, Yu Ru Kou, Yung-Pei Hsu, Low-Tone Ho. 2009. Resistin increases lipid accumulation by affecting class A scavenger receptor, CD36 and ATP-binding cassette transporter-A1 in macrophages. Life Sci. 84: 97-104.
31 Kuo-Hui Su, Jin-Yi Tsai, Yu Ru Kou, An-Na Chiang, Sheng-Huang Hsiao, Yuh-Lin Wu, Hsin-Han Hou, Ching-Chian Pan, Song-Kun Shyue, Tzong-Shyuan Lee*. 2009. Valsartan regulates the interaction of angiotensin II type 1 receptor and endothelial nitric oxide synthase via Src/PI3K/Akt signalling. Cardiovasc Res. 82:468-475. (*Corresponding author)
32 Chiu-Ling Hsu, Yuh-Lin Wu, Gau-Jun Tang, Tzong-Shyuan Lee*, Yu Ru Kou*. 2009. Ginkgo biloba extract confers protection from cigarette smoke extract-induced apoptosis in human lung endothelial cells: Role of heme oxygenase-1. Pulm Pharmacol Ther. 22:286-296. (*Corresponding author)
33 Tsai CY, Tzong-Shyuan Lee, Yu Ru Kou, Yuh-Lin Wu. 2009. Glucosamine inhibits IL-1b-mediated IL-8 production in prostate cancer cells by MAPK attenuation. J Cell Biochem. 108: 489-498.
34 Po-Ling Yu, Ching-I Wu, Tzong-Shyuan Lee, Wynn H.T. Pan, Paulus S. Wang, and Shyi-Wu Wang. 2009. Estradiol attenuated the reduction of striatal dopamine by amphetamine in ovariectomized rats. J Cell Biochem. 108: 1318-1324.
35 Yu Ru Kou, Tzong-Shyuan Lee. 2010. Intratracheal siRNA for the in vivo silencing of caspase-3: A novel therapy for acute lung injury? Crit Care Med. 38:1223-1224. (Editorial)
36 Kuo-Yun Lu, Li-Chieh Ching, Kuo-Hui Su, Yuan-Bin Yu, Yu Ru Kou, Sheng-Huang Hsiao, Yu-Chu Huang, Chien-Yu Chen, Li-Ching Cheng, Ching-Chian Pan, Tzong-Shyuan Lee*. 2010. Erythropoietin suppresses the formation of macrophage foam cells: Role of liver X receptor a. Circulation. 121: 1828-1837. (*Corresponding author)
37 Yuh-Lin Wu, Yu Ru Kou, Hui-Ling Ou, Han-Yun Chien, Kun-Han Chuang, Han-Hsun Liu, Tzong-Shyuan Lee, Cheng-Yen Tsai, Meng-Lun Lu. 2010. Glucosamine regulation of LPS-mediated inflammation in human bronchial epithelial cells. Eur J Pharmacol. 635: 219-226.
38 Tzong-Shyuan Lee*, Ching-Chian Pan, Chien-Chung Peng, Yu Ru Kou, Chien-Yu Chen, Li-Chieh Ching, Tsung-Huang Tsai, Ping-Chiang Lyu, Shu-Fen Chen, and Song-Kun Shyue*. 2010. Anti-atherogenic effect of berberine on LXRa-ABCA1-dependent cholesterol efflux in macrophages. J Cell Biochem. 111: 104-110. (*Corresponding author)
39 Jin-Yi Tsai, Kuo-Hui Su, Song-Kun Shyue, Yu Ru Kou, Yuan-Bin Yu, Sheng-Huang Hsiao, An-Na Chiang, Yuh-Lin Wu, LC Ching, Tzong-Shyuan Lee*. 2010. EGb761 ameliorates the formation of foam cells by regulating the expression of SR-A and ABCA1: Role of heme oxygenase-1. Cardiovasc Res. 88: 415-423. (*Corresponding author)
40 Tsung-Huang Tsai, Shu-Fen Chen, Tai-Yu Huang, Chun-Fu Tzeng, Ann-Shyn Chiang,Yu Ru Kou, Tzong-Shyuan Lee*, and Song-Kun Shyue*. 2011. Impaired CD14 and CD36 expression, bacterial clearance and toll-like receptor 4–MyD88 signaling in caveolin-1–deleted macrophages and mice. Shock. 35: 92-99. (*Corresponding author)
41 Tzu-Ming Wang, Chun-Jung Chen, Tzong-Shyuan Lee, Han-Yi Chao, Wen-Huey Wu, Shu-Chen Hsieh, Huey-Herng Sheu and An-Na Chianga. 2011. Docosahexaenoic acid attenuates VCAM-1 expression and NF-κB activation in TNF-a-treated human aortic endothelial cells. J Nutr Biochem. 22:187-194.
42 Wen-Chin Chiu, Chun-Jung Chen, Tzong-Shyuan Lee, Zit-Jie, Chen, Pei-Hsin Ke, and An-Na Chiang. 2011. Oxidative stress enhances AP-1 and NF-kB-mediated regulation of b2-glycoprotein I gene expression in hepatoma cells. J Cell Biochem. 111: 988-998.
43 Li-Ching Cheng, Kuo-Hui Su, Li-Chieh Ching, Song-Kun Shyue, Yu Ru Kou, Yuh-Lin Wu, Kuo-Yun Lu, Chien-Yu Chen, Ching-Chian Pan, Tzong-Shyuan Lee* . 2011. a-lipoic acid ameliorates foam cell formation via liver X receptor a-dependent up-regulation of ATP-binding cassette transporter A1 and G1. Free Radic. Biol. Med. 50: 47-54. (*Corresponding author)
44 Che-Feng Chang, Shu-Fen Chen, Tzong-Shyuan Lee, Hung-Fu Lee, Szu-Fu Chen, Song-Kun Shyue. 2011. Caveolin-1 Deletion Reduces Early Brain Injury after Experimental Intracerebral Hemorrhage. Am. J. Pathol. 178: 1749-1761.
45 Chun-Yueh Lin*, Tzong-Shyuan Lee*, Chin-Chang Chen, Cheng-An Chang, Yan-Jie Lin, Yung-Pei Hsu, Low-Tone Ho. 2011. Endothelin-1 exacerbates lipid accumulation by increasing the protein degradation of the ATP-binding cassette transporter G1 in macrophages. J Cell Physiol. 226:2198-2205. (*these authors contributed equally to this work).
46 Gau-Jun Tang, Hsin-Yi Wang, Jen-Ying Wang, Chih-Chieh Lee, Hsu-Wen Tseng, Yuh-Lin Wu, Song-Kun Shyue, Tzong-Shyuan Lee*, Yu Ru Kou*. The novel role of the AMP-activated protein kinase signaling in the induction of IL-8 in human lung epithelial cells and lung inflammation in mice by cigarette smoke. Free Radic. Biol. Med. 50:1492-502.
47 Li-Chieh Ching, Yu Ru Kou, Song-Kun Shyue, Kuo-Hui Su, Jeng Wei, Li-Ching Cheng, Yuan-Bin Yu, Ching-Chian Pan, Tzong-Shyuan Lee*. 2011. Molecular mechanisms of activation of endothelial nitric oxide synthase mediated by endothelial transient receptor potential vanilloid type 1. Cardiovasc Res. 91:492-501. (*Corresponding author)
48 Chi-Huei Chiang, Chiao-Hui Chuang, Shiou-Ling Liu, Tzong-Shyuan Lee, Yu Ru Kou, Haibo Zhang. 2011. Apocynin attenuates ventilator-induced lung injury in an isolated and perfused rat lung model. Intensive Care Med. 37:1360-1367.
49 Kuo-Hui Su, Song-Kun Shyue, Li-Chieh Ching, Yu Ru Kou, An-Na Chiang, Yuan-Bin Yu, Chien-Yu Chen, Ching-Chian Pan, Tzong-Shyuan Lee*. 2011. b common receptor integrates the erythropoietin signaling in activation of endothelial nitric oxide synthase. J Cell Physiol. 226:3330-3339. (*Corresponding author)
50 Tzong-Shyuan Lee, Yu Ru Kou. 2011. How alcohol impairs the granulocyte expansion during septicemia. Crit Care Med. 39: 2194-2195. (Editorial)
51 Chien-Yu Chen, Li-Chieh Ching, Song-Kun Shyue, An-Na Chiang, Yuh-Lin Wu, Yu Ru Kou, Ching-Chian Pan, Tzong-Shyuan Lee*. 2011. Wogonin promotes cholesterol efflux by increasing protein phosphatase 2B-dependent dephosphorylation on ATP-binding cassette transporter-A1 in macrophage foam cells. J Nutr Biochem. 22:1015-1021. (*Corresponding author)
52 Hsin-Han Hou, Bruce D. Hammock, Kou-Hui Su, Yu Ru Kou, Christophe Morisseau, Susumu Imaoka, Ami Oguro, Song-Kun Shyue, Jin-Feng Zhao, and Tzong-Shyuan Lee*. 2012. N-terminal domain of soluble epoxide hydrolase negatively regulates the VEGF-mediated activation of endothelial nitric oxide synthase. Cardiovasc Res. 93:120-129. (*Corresponding author)
53 Tzong-Shyuan Lee, Yu Ru Kou. 2012. Enhancing endothelial progenitor cell therapy for critical limb ischemia by extracorporeal shock wave. Crit Care Med. 40:332-333. (Editorial)
54 Yu-Wen Hung, Sing-I Yang, Pei-Yu Huang, Tzong-Shyuan Lee, Terry B.J. Kuo, Chun-Hing Yiu, Yang-Hsin Shih, Yung-Yang Lin. 2012. The duration of sustained convulsive seizures determines the pattern of hippocampal neurogenesis and the development of spontaneous epilepsy in rats. Epilepsy Research. 98:206-215.
55 Kuo-Hui Su, Yuan-Bin Yu, Hsin-Han Hou, Jin-Feng Zhao, Yu Ru Kou, Li-Ching Cheng, Song-Kun Shyue, Tzong-Shyuan Lee*. 2012. AMP-activated protein kinase mediates erythropoietin-induced activation of endothelial nitric oxide synthase. J Cell Physiol. 27: 3053-3062. (*Corresponding author)
56 Jing-Feng Zhao, Li-Chieh Ching, Yu-Chu Huang, Chien-Yu Chen, An-Na Chiang, Yu Ru Kou, Song-Kun Shyue, Tzong-Shyuan Lee*. 2012. Molecular mechanism of curcumin on the suppression of cholesterol accumulation in macrophage foam cells and atherosclerosis. Mol Nutr Food Res. 56:691-701. (*Corresponding author)
57 Hung-Fu Lee, Tzong-Shyuan Lee*, Yu Ru Kou*. 2012. Anti-inflammatory and neuroprotective effects of triptolide on traumatic brain injury in rats. Respiratory Physiology & Neurobiology. 182: 1-8. (*Corresponding author).
58 Yi-Jen Liao, Tzu-Lang Chen, Tzong-Shyuan Lee, Hsiang-An Wang, Chung-Kwe Wang, Li-Ying Liao, Ren-Shyan Liu, Shiu-Feng Huang, and Yi-Ming Arthur Chen. 2012. Glycine N-methyltransferase deficiency affects Niemann-Pick type C2 protein stability and regulates hepatic cholesterol homeostasis. Molecular Medicine. 18:412-422.
59 Yuan-Bin Yu, Yu-Wen Liao, Kou-Hui Su, Tsung-Ming Chang, Song-Kun Shyue, Yu Ru Kou*, Tzong-Shyuan Lee*. 2012. Prior exercise training alleviates the lung inflammation induced by subsequent exposure to environmental cigarette smoke. Acta Physiologica (Oxford). 205: 532-540. (*Corresponding author)
60 Chien-Yu Chen, Li-Chieh Ching, Yi-Jen Liao, Yuan-Bin Yu, Chia-Yuan Tsou, Song-Kun Shyue, Yi-Ming Arthur Chen*, Tzong-Shyuan Lee*. 2012. Deficiency of glycine N-methyltransferase aggravates atherosclerosis in apolipoprotein E-null mice. Molecular Medicine. 18: 744-752. (*Corresponding author)
61 Yu-Cheng Chang, Tzong-Shyuan Lee, An-Na Chiang. 2012. Quercetin enhances ATP-binding cassette transporter A1 expression and cholesterol efflux through a p38-dependent pathway in macrophages. J Lipid Res. 53: 1840-1850.
Li-Chieh Ching, Chien-Yu Chen, Kuo-Hui Su, Hsin-Han Hou, Song-Kun Shyue, Yu Ru Kou, Tzong-Shyuan Lee*. 2012. Implication of AMP-activated protein kinase in transient receptor potential vanilloid type 1-mediated activation of endothelial nitric oxide synthase. Molecular Medicine. 18: 805-815. (*Corresponding author)
63 Yu YB, Gau JP, Liu CY, Yang MH, Chiang SC, Hsu HC, Hong YC, Hsiao LT, Liu JH, Chiou TJ, Chen PM, Tzong-Shyuan Lee, Chou LF, Tzeng CH, Chen TJ. 2012. A nation-wide analysis of venous thromboembolism in 497,180 cancer patients with the development and validation of a risk-stratification scoring system. Thromb Haemost. 108: 225-235.
64 Wen-Chin Chiu, Jan-Yu Lin, Tzong-Shyuan Lee, Li-Ru You, An-Na Chiang. 2013. b2-glycoprotein I inhibits VEGF-induced proliferation and migration of human aortic endothelial cells and suppresses phosphorylation of VEGFR2, ERK1/2 and Akt. Mol Cell Biochem. 372: 9-15.
65 Ko-Lin Kuo, Szu-Chun Hung, Yao-Ping Lin, Ching-Fang Tang, Tzong-Shyuan Lee, Chih-Pei Lin, Der-Cherng Tarng. 2013. Intravenous ferric chloride hexahydrate, a pro-oxidant, affects endothelial function and cardiovascular outcome in chronic hemodialysis patients. PLos One. 7: e50295.
66 Diahn-Warng Perng, Tsung-Ming Chang, Jen-Ying Wang, Chih-Chieh Lee, Shing-Hwa Lu, Song-Kun Shyue, Tzong-Shyuan Lee*, Yu Ru Kou*. 2013. Inflammatory role of AMP-activated protein kinase signaling in experimental model of wood-smoke inhalation injury. Crit Care Med. 41: 120-132. (*Corresponding author)
67 Jeng Wei, Li-Chieh Ching, Jin-Feng Zhao, Song-Kun Shyue, Yu Ru Kou, Tzong-Shyuan Lee*. 2013. Essential role of transient receptor potential vanilloid type 1 in the evodiamine-mediated protection against atherosclerosis in apolipoprotein E deficient mice. Acta Physiologica (Oxford). 207:299-307. (*Corresponding author)
68 Jin-Feng Zhao, Li-Chieh Ching, Yu Ru Kou, Shing-Jong Lin, Jeng Wei, Song-Kun Shyue*, Tzong-Shyuan Lee*. 2013. Activation of TRPV1 prevents oxLDL-induced lipid accumulation and TNF-a-induced inflammation in macrophages: Role of liver X receptor. Mediators of Inflammation. 2013:925171 (*Corresponding author)
69 Li-Chieh Ching, Jin-Feng Zhao, Kuo-Hui Su, Song-Kun Shyue, Chiao-Po Hsu, Tse-Min Lu, Shing-Jong Lin, Tzong-Shyuan Lee*. 2013. Activation of transient receptor potential vanilloid 1 decreases endothelial nitric oxide synthase phosphorylation at Thr497 by protein phosphatase 2B-dependent dephosphorylation of protein kinase C. Acta Physiologica. 209:124-135. (*Corresponding author)
70 Jin-Feng Zhao, Shr-Jeng Jim Leu, Song-Kun Shyue, Kuo-Hui Su, Jeng Weid*, Tzong-Shyuan Lee*. 2013. Novel effect of paeonol on the formation of foam cells: Promotion of LXRa-ABCA1-dependent cholesterol efflux in macrophages. Am J Chin Med. 41:1079-1096. (*Corresponding author)
71 Jin-Feng Zhao, Song-Kun Shyue, Shing-Jong Lin, Jeng Wei*, Tzong-Shyuan Lee*. 2014. Excess nitric oxide impairs liver X receptor a-ATP-binding cassette transporter A1-dependent cholesterol efflux in macrophage foam cells. J Cell Physiol. 229:117-125. (*Corresponding author)
72 Wei-Wen Chen, Chen-Hao Chien, Chiung-Lin Wang, Huai-Hsien Wang, Yuh-Lin Wang, Shih-Torng Ding, Tzong-Shyuan Lee, Ta-Chau Chang. 2013. Automated quantitative analysis of lipid accumulation and hydrolysis in living macrophages with label-free imaging. Anal Bioanal Chem. 405:8549-8559.
73 Hsin-Kuo Ko, Wen-Hu Hsu, Chin-Cheng Hsieh, Te-Cheng Lien, Tzong-Shyuan Lee, Yu Ru Kou. 2014. High expression of high- mobility group box 1 in the blood and lungsis associated with the development of chronic obstructive pulmonary disease in smokers. Respirology 19:253-261.
74 Wen-Yueh Chou, Jin-Feng Zhao, Yi-Ming Arthur Chen, Kuan-I Lee, Kuo-Hui Su, Song-Kun Shyue, Tzong-Shuan Lee*. 2014. Role of glycine N-methyltransferase in experimental ulcerative colitis. J Gastroenterol Hepatol. 29:494-501. (*Corresponding author)
75 Chia-Yuan Tsou, Chien-Yu Chen, Jin-Feng Zhao, Kuo-Hui Su, Hsueh-Te Lee, Shing-Jong Lin, Song-Kun Shyue, Sheng-Huang Hsiao*, Tzong-Shyuan Lee*. 2014. Activation of soluble guanylyl cyclase prevents foam cell formation and atherosclerosis. Acta Physiologica. 210: 799-810. (*Corresponding author)
76 Yuh-Lin Wu, An-Hsuan Lin, Chao-Hung Chen, Wen-Chien, Huang, Hsin-Yi Wang, Meng-Han Liu, Tzong-Shyuan Lee*, Yu Ru Kou*. 2014. Glucosamine attenuates cigarette smoke induced-lung inflammation via its antioxidant function and inhibition of ROS-sensitive inflammatory signaling. Free Radic. Biol. Med. 69: 208-218. (*Corresponding author)
77 Kuo-Hui Su, Kuan-I Lee, Song-Kun Shyue, Jeng Wei, Tzong-Shyuan Lee*. 2014. Implication of transient receptor potential vanilloid type 1 in 14,15-epoxyeicosatrienoic acid-induced angiogenesis. International Journal of Biological Science. 10: 990-996. (*Corresponding author)
78 Meng-Han Liu, An-Hsuan Lin, Hung-Fu Lee, Hsin-Kuo Ko, Tzong-Shyuan Lee*, Yu Ru Kou*. 2014. Paeonol attenuates cigarette smoke-induced lung inflammation by inhibiting ROS-sensitive inflammatory signaling. Mediators of Inflammation. 2014:651890  (*Corresponding author)
79 Kuo-Hui Su, Shing-Jong Lin, Jeng Wei, Sheng-Huang Hsiao, Kuan-I Lee, Jin-Feng Zhao, Song-Kun Shyue*, Tzong-Shyuan Lee*. 2014. The essential role of transient receptor potential vanilloid 1 in simvastatin-induced activation of endothelial nitric oxide synthase and angiogenesis. Acta Physiologica. 212: 191–204. (*Corresponding author)
80 Kuo-Hui Su, Shing-Jong Lin, Jeng Wei, Sheng-Huang Hsiao, Kuan-I Lee, Jin-Feng Zhao, Song-Kun Shyue*,Tzong-Shyuan Lee*. 2014. The essential role of transient receptor potential vanilloid 1 in simvastatin-induced activation of endothelial nitric oxide synthase and angiogenesis. Acta Physiologica. 5:440
81 Meng-Han Liu, An-Hsuan Lin, Shing-Hwa Lu, Ruo-Yun Peng, Tzong-Shyuan Lee*, Yu Ru Kou*. 2014. Eicosapentaenoic acid attenuates cigarette smoke-induced lung inflammation by inhibiting ROS-sensitive inflammatory signaling. Frontiers in Physiology: Respiratory Physiology. 5: 440.
82 Yu-Wen Hung, Shao-Wen Hung, Yi-Chen Wu, Lin-King Wong, Ming-Tsong Lai, Yang-Hsin Shih, Tzong-Shyuan Lee*, Yung-Yang Lin*. 2015. Soluble epoxide hydrolase activity regulates inflammatory responses and seizure generation in two mouse models of temporal lobe epilepsy. Brain, Behavior, and Immunity. 43: 118-129. (*Corresponding author)
83 Kuan-Chuan Pao, Jin-Feng Zhao, Tzong-Shyuan Lee, Ying-Pei Huang, Chien-Chung Han, Lin-Chiang Sherlock Huang, Ming-Hua Hsu*. 2015. Low-dose paeonol derivatives alleviate lipid accumulation. RSC Advances 5:5652-5656.
84 Hsin-Kuo Ko, Hung-Fu Lee, An-Hsuan Lin, Meng-Han Liu, Ching-I Liu, Tzong-Shyuan Lee*, Yu Ru Kou*. 2015. Regulation of cigarette smoke induction of IL-8 in macrophages by AMP-activated protein kinase signaling: Role of nAChRs and ROS. J Cell Physiol. 230: 1781-1793. (*Corresponding author)
85 Chin-Wei Chiang, Hsueh-Te Lee, Der-Cherng Tarng, Ko-Lin KuoLi-Ching Cheng, Tzong-Shyuan Lee*. 2015. Deletion of soluble epoxide hydrolase attenuates inflammation and fibrosis in experimental obstructive nephropathy. Mediators of Inflammation. 2015:693260. (*Corresponding author)
86 Bei-Chia Guo, Jeng Wei, Kuo-Hui Su, An-Na Chiang, Li-Ching Cheng, Jin-Feng Zhao, Song-Kun Shyue, Tzong-Shyuan Lee*. 2015. Transient receptor potential vanilloid type 1 is vital in (-)-epigallocatechin-3-gallate-mediated activation of endothelial nitric oxide synthase. Mol Nutr Food Res. 59: 646-657. (*Corresponding author)
87 Tzong-Shyuan Lee*, Kuo-Yun Lu, Yuan-Bin Yu, Hsueh-Te Lee, Feng-Chuan Tsai. 2015.  common receptor mediates erythropoietin-conferred suppression on oxLDL-induced lipid accumulation and inflammation in macrophages. Mediators of Inflammation. 2015:439759. (*Corresponding author)
88 Hsin-Han Hou, Yi-Jen Liao, Sheng-Huang Hsiao, Song-Kun Shyue, Tzong-Shyuan Lee*. 2015. Role of phosphatase activity of soluble epoxide hydrolase in regulating simvastatin-activated endothelial nitric oxide synthase. Scientific Reports 5:13524. (*Corresponding author).
89 An-Hsuan Lin, Meng-Han Liu, Hsin-Kuo Ko, Diahn-Warng Perng, Tzong-Shyuan Lee* and Yu Ru Kou*. 2015. Lung epithelial TRPA1 transduce the extracellular ROS into transcriptional regulation of lung inflammation induced by cigarette smoke: the role of influxed Ca2+. Mediators of Inflammation. 2015:148367. (*Corresponding author)
90 Han-Fang Wu, Hsin-Ju Yen, Chi-Chen Huang, Yi-Chao Lee, Su-Zhen Wu, Tzong-Shyuan Lee, Hui-Ching Lin. 2015. Soluble epoxide hydrolase inhibitor enhances synaptic neurotransmission and plasticity in mouse prefrontal cortex. J Biomed Sci. 22:94.
91 Jin-Feng Zhao, Song-Kun Shyue, Tzong-Shyuan Lee*. 2016. Excess nitric oxide activates TRPV1-Ca2+-calpain signaling and promotes the PEST-dependent degradation of liver X receptor International Journal of Biological Sciences 12:18-29.
92 Jin-Feng Zhao, Sheng-Huang Hsiao, Ming-Hua Hsu, Kuan-Chuan Pao, Yu Ru Kou, Song-Kun Shyue, Tzong-Shyuan Lee*. 2016. Di-(2-ethylhexyl) phthalate accelerates atherosclerosis in apolipoprotein E-deficient mice. Archives of Toxicology. 90: 181-190. (*Corresponding author)
93 Kuan-I Lee, Chin-Wei Chiang, Hui-Ching Lin, Jin-Feng Zhao, Cheng-Ta Li, Song-Kun Shyue, Tzong-Shyuan Lee*. 2016. Maternal di-(2-ethylhexyl) phthalate exposure deregulates blood pressure, adiposity, cholesterol metabolism and social interaction in offspring. Archives of Toxicology. 90:1211-1224. (*Corresponding author)
94 Chiao-Po Hsu, Jin-Feng Zhao, Shing-Jong Lin, Song-Kun Shyue, Bei-Chia Guo, Tse-Min Lu*, Tzong-Shyuan Lee*. 2016. Asymmetric dimethylarginine limits the efficacy of simvastatin activating endothelial nitric oxide synthase. J Am Heart Assoc. 5: e003327. (*Corresponding author)
95 Kuan-I Lee, Hsueh-Te Lee, Hui-Ching Lin, Huey-Jen Tsay, Feng-Chuan Tsai, Song-Kun Shyue, Tzong-Shyuan Lee*. 2016. Transient receptor potential ankyrin 1 channels in Alzheimer’s disease. Journal of Neuroinflammation. 13:92. (*Corresponding author)
96 Jin-Feng Zhao, Song-Kun Shyue, Yu Ru Kou, Tse-Min Lu*, Tzong-Shyuan Lee*. Transient receptor potential ankyrin 1 channel involved in atherosclerosis and macrophage-foam cell formation. 2016. International Journal of Biological Sciences (In Press). (*Corresponding author)
97 Wei-Ming Chen, Wayne H-H Sheu, Pei-Chi Tseng, Tzong-Shyuan Lee, Wen-Jane Lee, Pey-Jium Chang, Chiang. 2016. Modulation of microRNA expression in subjects with metabolic syndrome and decrease of cholesterol efflux from macrophages via microRNA-33-mediated attenuation of ATP-binding cassette transporter A1 expression by statins. PLOS ONE (In Press).
98 Kuan-I Lee, Hui-Ching Lin, Hsueh-Te Lee, Feng-Chuan Tsai, Tzong-Shyuan Lee*. 2016. Loss function of transient receptor potential ankyrin 1 channels deregulates emotional, social, cognitive, learning and memory development. Molecular Neurobiology (In Press). (*Corresponding author)
99 Yuan-Bin Yu, Kuo-Hui Su, Bei-Chia Guo, Kuan-I Lee, Jeng Wei, Tzong-Shyuan Lee. 2016. Transient receptor potential vanilloid 1 in regulating erythropoietin-induced activation of endothelial nitric oxide synthase. Acta Physiologica (In Press). (*Corresponding author)
100 Yuh-Ching Twu, Tzong-Shyuan Lee, Yun-Lian Lin, Shih-Ming Hsu, Yuan-Hsi Wang, Chia-Yu Liao, Chung-Kwe Wang, Yu-Chih Liang, Yi-Jen Liao. 2016. Niemann-Pick type C2 protein mediates hepatic stellate cells activation by regulating free cholesterol accumulation. Int J Mol Sci. 17: 1122.
101 Yi-Jen Liao, Tzong-Shyuan Lee, Yuh-Ching Twu, Ching-Ping Yang, Chung-Kwe Wang, Shih-Ming Hsu, Yu-Chih Liang, and Yi-Ming Chen. 2016. Glycine N-methyltransferase deficiency accumulate triglycerides and impair insulin signaling by modulating PI3K/Akt pathway in the liver. J Biomed Sci (In Press).
102 Tse-Min Lu, Chiao-Po Hsu, Chao-Fu Chang, Chih-Ching Lin, Tzong-Shyuan Lee, Shing-Jong Lin, Wan-Leong Chan. Asymmetric dimethylarginine predicts the risk of contrast-induced acute kidney injury in patients undergoing cardiac catheterization. Atherosclerosis (In Press).
Research grant: 
Lab membership:




Voice Play