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National Yang-Ming University, Department & Institute of Physiology
National Yang-Ming University, Department & Institute of Physiology
Eileen Jea Chien

 Research Field    Publications    Research grant    Lab intro    (2013.11.29  update)


Ai-Hwa Jea also known as Eileen Jea Chien

Position Professor
Tel. 886-2-2826-7088
Fax No. 886-2-2826-4049

1989 PhD

Department of Physiology & Biophysics, Albert Einstein College of Medicine, New York, NY 10641, USA

1978 MS

Department of Biology, National Taiwan Normal University

1975 BS Department of Biology, National Taiwan Normal University

Research Interests:

My major research interest is cell physiology. These include 1) mitogenic signals in  human peripheral T cells, 2) how endotoxin (LPS) stimulating T cells, 3) nongenomic effects of progesterone in T cells. T cells were isolated from human peripheral blood. The intracellular calcium ([Ca2+]i) and intracellular pH (pHi) were measured using, respectively, the fluorescent dyes, Fura-2 and BCECF. ELISA was used to analyze cytokines (IL-2, IL-4, INFγ…etc.). The degree of proliferation was determined by [3H]-thymidine incorporation into T cells. The expression of surface markers, CD25, CD28, and CTLA-4 were detected by flow cytometry. Eventually, the techniques in molecular biology will be employed in my future studies. At present, I have 3 master students and 1 Ph.D. student to share my research work, and we enjoy the projects to explore the miracle of life science!


Selected Recent Publications:

1 Chen JJ, Lin DJ, Liu MS, Chien EJ. Non-genomic rapid responses via progesterone in human peripheral T cells are not indirectly mimicked by sphingosine 1-phosphate. Steroids. 2013 Nov 20. [Epub ahead of print]
2 Lin DJ, Hsieh DJY, Chen JJ, Lin VHC, Tsai YL and Chien EJ. Inhibition of progesterone receptor agonist R5020 on Na+/H+-exchange 1 activity and the proliferative activation of human T cells. Adaptive Medicine 5:70-76, 2013.
3 Lai JN, Wang OYH, Lin VHC, Liao CF, Tarng DC, Chien EJ. The non-genomic rapid acidification in peripheral T cells by progesterone depends on intracellular calcium increase and not on Na+/H+-exchange inhibition. (Accept) Steroids, RRSH Special Issue 2012.
4 Chang CP, Wang SW, Huang ZL, Wang OY, Huang MI, Lu LM, Tarng DC, Chien CH, Chien EJ. Non-genomic rapid inhibition of Na+/H+-exchange 1 and apoptotic immunosuppression in human T cells by glucocorticoids. J Cell Physiol. 223:679-686, 2010.
5 CH Chien, JN Lai, CF Liao, OY Wang, LM Lu, MI Huang, WF Lee, MC Shie and EJ Chien. Mifepristone acts as progesterone antagonist of non-genomic responses but inhibits phytohemagglutinin induced proliferation in human T cells. Hum Reprod. 24(8):1968-1975,2009.
6 Chien EJ, Liao CF, Chang CP,Pu HF,Lu LM, Shie MC,  Hsieh DJ and Hsu MT. The Non-genomic effects on Na+/H+-exchange 1 by progesterone and 20α-hydroxyprogesterone in human T cells. J Cell Physiol. 211:544-550, 2007.
7 Chien EJ, Chang CP, Lee WF, Su TH, Wu CH. Non-genomic immunosuppressive actions of rogesterone inhibits PHA-induced alkalinization and activation in T cells. J Cell Biochem. 99:292-304, 2006.
8 Chien EJ, Chen CC, Chien CH, Yeh TP, Lu LM. Activation and up-regulation of phospholipase D expression by lipopolysaccharide in human peripheral T cells. Chin J Physiol.47:203-9, 2004.


Research grant: 


Lab membership:

Master Student:  

Ph.D. Student: Hui-Chen Lin, Shuei-Yu Liou

Research Assistant:

Post Doctoral Fellows:




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