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National Yang-Ming University, Department & Institute of Physiology

National Yang-Ming University, Department & Institute of Physiology

Eileen Jea Chien

 Research Field    Publications    Research grant    Lab intro    (2013.11.29  update)

Name

Ai-Hwa Jea also known as Eileen Jea Chien

 
Position Professor
Tel. 886-2-2826-7088
Fax No. 886-2-2826-4049
E-mail

eileen@ym.edu.tw

Education
1989 PhD

Department of Physiology & Biophysics, Albert Einstein College of Medicine, New York, NY 10641, USA

1978 MS

Department of Biology, National Taiwan Normal University

1975 BS Department of Biology, National Taiwan Normal University
Experience

Research Interests:

My major research interest is cell physiology. These include 1) mitogenic signals in  human peripheral T cells, 2) how endotoxin (LPS) stimulating T cells, 3) nongenomic effects of progesterone in T cells. T cells were isolated from human peripheral blood. The intracellular calcium ([Ca2+]i) and intracellular pH (pHi) were measured using, respectively, the fluorescent dyes, Fura-2 and BCECF. ELISA was used to analyze cytokines (IL-2, IL-4, INFγ…etc.). The degree of proliferation was determined by [3H]-thymidine incorporation into T cells. The expression of surface markers, CD25, CD28, and CTLA-4 were detected by flow cytometry. Eventually, the techniques in molecular biology will be employed in my future studies. At present, I have 3 master students and 1 Ph.D. student to share my research work, and we enjoy the projects to explore the miracle of life science!

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Selected Recent Publications:

1 Chen JJ, Lin DJ, Liu MS, Chien EJ. Non-genomic rapid responses via progesterone in human peripheral T cells are not indirectly mimicked by sphingosine 1-phosphate. Steroids. 2013 Nov 20. [Epub ahead of print]
2 Lin DJ, Hsieh DJY, Chen JJ, Lin VHC, Tsai YL and Chien EJ. Inhibition of progesterone receptor agonist R5020 on Na+/H+-exchange 1 activity and the proliferative activation of human T cells. Adaptive Medicine 5:70-76, 2013.
3 Lai JN, Wang OYH, Lin VHC, Liao CF, Tarng DC, Chien EJ. The non-genomic rapid acidification in peripheral T cells by progesterone depends on intracellular calcium increase and not on Na+/H+-exchange inhibition. (Accept) Steroids, RRSH Special Issue 2012.
4 Chang CP, Wang SW, Huang ZL, Wang OY, Huang MI, Lu LM, Tarng DC, Chien CH, Chien EJ. Non-genomic rapid inhibition of Na+/H+-exchange 1 and apoptotic immunosuppression in human T cells by glucocorticoids. J Cell Physiol. 223:679-686, 2010.
5 CH Chien, JN Lai, CF Liao, OY Wang, LM Lu, MI Huang, WF Lee, MC Shie and EJ Chien. Mifepristone acts as progesterone antagonist of non-genomic responses but inhibits phytohemagglutinin induced proliferation in human T cells. Hum Reprod. 24(8):1968-1975,2009.
6 Chien EJ, Liao CF, Chang CP,Pu HF,Lu LM, Shie MC,  Hsieh DJ and Hsu MT. The Non-genomic effects on Na+/H+-exchange 1 by progesterone and 20α-hydroxyprogesterone in human T cells. J Cell Physiol. 211:544-550, 2007.
7 Chien EJ, Chang CP, Lee WF, Su TH, Wu CH. Non-genomic immunosuppressive actions of rogesterone inhibits PHA-induced alkalinization and activation in T cells. J Cell Biochem. 99:292-304, 2006.
8 Chien EJ, Chen CC, Chien CH, Yeh TP, Lu LM. Activation and up-regulation of phospholipase D expression by lipopolysaccharide in human peripheral T cells. Chin J Physiol.47:203-9, 2004.

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Research grant: 

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Lab membership:

Master Student:  

Ph.D. Student: Hui-Chen Lin, Shuei-Yu Liou

Research Assistant:

Post Doctoral Fellows:

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